Tylenol and Glutathione: A Closer Look At Their Relationship A Possible Antidote for Tylenol (Paracetamol) Overdose American Council on Science and Health Best Glutathione Supplement 4 Recommendations in 2026 Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors.