Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Glutathione Understanding the Powerful Master Antioxidant Glutathione: Master Antioxidant, Reducing Oxidative Stress, and Detoxification Glutathione depletion and dual model oxygen balance disruption for photodynamic therapy enhancement ScienceDirect